4. 3 L stirred tank bioreactor vessel.

5. Bio-welder.

6. pH probe (see Note 8).

7. DO probe.

8. Temperature probe.

9. Motor.

10. Heating jacket.

11. Hemostat.

12. Parafilm.

13. Conical tubes.

14. Syringes.

15. Extension sets.

16. Junction box.

17. Bioprocessing system.

18. Analytical scale.

19. Waste bag.

20. Mesh filter.

21. Intravenous pole.

22. Peristaltic pump.

23. Cell counter (hemocytometer or automated).

Fig. 3 Assemble the harvest line extension. This allows for the harvest or collection of expanded cells from the

bioreactor vessel

42

Marites T. Woon et al.